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Sina Ghaemmaghami

Sina Ghaemmaghami

  • Associate Professor
  • Director of Undergraduate Research in the College


326A Hutchison
(585) 275-4829

Office Hours: By appointment


Research Overview

Our laboratory studies the mechanisms of protein folding and degradation within cells. We investigate how cells maintain a balance between these two processes, and how this homeostasis is affected by disease and aging. The projects in the lab draw on a number of disciplines including protein biochemistry, proteomics and computational biology. Current research areas include:

• development of proteomic methodologies for global analysis of cellular protein degradation
• uncovering the mechanism of selectivity in macroautophagy
• analysis of the kinetics and efficiency of cellular protein folding
• mechanistic studies of prion aggregate formation and characterization of novel anti-prion compounds

More information can be found at


Publications can be found at

Selected Publications

  • Ghaemmaghami S. (2016) Biology and genetics of PrP prion strains. Cold Spring Harb Perspect Med. 2016 doi: 10.1101/cshperspect.a026922
  • Welle KA, Zhang T, Hyrohorenko JR, Shen S, Qu J, Ghaemmaghami S. (2016) Time-resolved analysis of proteome dynamics by TMT-SILAC hyperplexing. 2016 Oct 20. [Epub ahead of print]
  • Zhang T, Ghaemmaghami S. (2016) Auophagic Punctum: Global analysis of cellular protein flux quantifies the selectivity of basal autophagy. Autophagy. 2016 Aug 2;12(8):1411-2.
  • Zhang T, Shen S, Qu J, Ghaemmaghami S. (2016) Global analysis of cellular protein flux quantifies the selectivity of basal autophagy. Cell Rep. 2016 Mar 15;14(10):2426-39.
  • Shen S, Li J, Hilchey S, Shen X, Tu C, Qiu X, Ng A, Ghaemmaghami S, Wu H, Zand MS, Qu J. (2016) Ion-current-based temporal proteomic profiling of influenza-A-virus-infected mouse lungs revealed underlying mechanisms of altered integrity of the lung microvascular barrier. J Proteome Res. 2016 Feb 5;15(2):540-53
  • Zhang T, Nouri E, Li J, Price JC, Hellerstein M, Qu J, Ghaemmaghami S. (2014) Kinetics of precursor labeling in SILAC experiments Anal. Chem. (Epub ahead of print – PMID:25301408)
  • Ghaemmaghami S, Russo M, Renslo AR. (2014) Successes and Challenges in Phenotype-Based Lead Discovery for Prion Diseases J. Med. Chem. Aug 28;57(16):6919-29.
  • Price JC, Ghaemmaghami S. (2014) Analysis of proteome dynamics in mice by isotopic labeling. Methods. Mol. Biol. 1156:111-31.
  • Miller-Vedam L, Ghaemmaghami S. (2013) Strain Specificity and Drug Resistance in Anti-Prion Therapy. Cur. Top. Med. Chem. 13(19):2397-406.
  • Silber BM, Gever JL, Li Z, Gallardo-Godoy A, Renslo AR, Widjaja K, Irwin JJ, Rao S,  Jacobson MP, Ghaemmaghami S, Prusiner SB. (2013) Antiprion compounds that reduce PrPSc levels in dividing and stationary-phase cells. Bioorgan. Med. Chem. 15;21(24):7999-8012.
  • Ghaemmaghami S, Colby DW, Nguyen HO, Hayashi S, Oehler A, DeArmond SJ, Prusiner SB. (2013) Convergent replication of mouse synthetic prion strains. Am J Pathol. 182(3):866-74.
  • Ahn M, Ghaemmaghami S, Huang Y, Phuan PW, May BC, Giles K, Dearmond SJ, Prusiner SB. (2012) Pharmacokinetics of Quinacrine Efflux from Mouse Brain via the P-glycoprotein Efflux Transporter. PLoS One. 7(7):e39112.
  • Guan S, Price JC, Ghaemmaghami S, Prusiner SB, Burlingame AL (2012) Compartment modeling for mammalian protein turnover studies by stable isotope metabolic labeling. Anal. Chem. Mar 23. May 1;84(9):4014-21.
  • Friberg KN, Hung G, Wancewicz E, Giles K, Black C, Freier S, Bennett F, DeArmond SJ, Freyman Y, Lessard P, Ghaemmaghami S, Prusiner SB (2012) Intracerebral Infusion of Antisense Oligonucleotides Into Prion-infected Mice. Mol. Ther. Nucleic Acids  1, e9; doi:10.1038/mtna.2011.6
  • Guan S, Price JC, Prusiner SB, Ghaemmaghami S, Burlingame AL. A data processing pipeline for mammalian proteome dynamics studies using stable isotope metabolic labeling. Mol Cell Proteomics. 2011 Sep 21. [Epub ahead of print] PubMed PMID: 21937731.
  • Poncet-Montange G, St Martin SJ, Bogatova OV, Prusiner SB, Shoichet BK, Ghaemmaghami S. A survey of antiprion compounds reveals the prevalence of non-PrP molecular targets. J Biol Chem. 2011 Aug 5;286(31):27718-28. Epub 2011 May 24. PubMed PMID: 21610081; PubMed Central PMCID: PMC3149362.
  • Ghaemmaghami S, Watts JC, Nguyen HO, Hayashi S, Dearmond SJ, Prusiner SB. Conformational Transformation and Selection of Synthetic Prion Strains. J Mol Biol. 2011 Aug 4. [Epub ahead of print] PubMed PMID: 21839745.
  • Price JC, Guan S, Burlingame A, Prusiner SB, Ghaemmaghami S. Analysis of proteome dynamics in the mouse brain. Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14508-13. PubMed PMID: 20699386; PubMed Central PMCID: PMC2922600.
  • Ghaemmaghami S, Ullman J, Ahn M, St Martin S, Prusiner SB. Chemical induction of misfolded prion protein conformers in cell culture. J Biol Chem. 2010 Apr 2;285(14):10415-23. Epub 2009 Dec 2. PubMed PMID: 19955177; PubMed Central PMCID: PMC2856248.
  • Ghaemmaghami S, May BC, Renslo AR, Prusiner SB. Discovery of 2-aminothiazoles as potent antiprion compounds. J Virol. 2010 Apr;84(7):3408-12. Epub 2009 Dec 23. PubMed PMID: 20032192; PubMed Central PMCID: PMC2838138.
  • Ghaemmaghami S, Ahn M, Lessard P, Giles K, Legname G, DeArmond SJ, Prusiner SB. Continuous quinacrine treatment results in the formation of drug-resistant prions. PLoS Pathog. 2009 Nov;5(11):e1000673. Epub 2009 Nov 26. PubMed PMID:19956709; PubMed Central PMCID: PMC2777304.
  • Ingolia NT, Ghaemmaghami S, Newman JR, Weissman JS. Genome-wide analysis in vivo of translation with nucleotide resolution using ribosome profiling. Science. 2009 Apr 10;324(5924):218-23. Epub 2009 Feb 12. PubMed PMID: 19213877; PubMed Central PMCID: PMC2746483.
  • Ghaemmaghami S, Phuan PW, Perkins B, Ullman J, May BC, Cohen FE, Prusiner SB. Cell division modulates prion accumulation in cultured cells. Proc Natl Acad Sci U S A. 2007 Nov 13;104(46):17971-6. Epub 2007 Nov 7. PubMed PMID: 17989223; PubMed Central PMCID: PMC2084281.
  • Newman JR, Ghaemmaghami S, Ihmels J, Breslow DK, Noble M, DeRisi JL, Weissman JS. Single-cell proteomic analysis of S. cerevisiae reveals the architecture of biological noise. Nature. 2006 Jun 15;441(7095):840-6. Epub 2006 May 14. PubMed PMID: 16699522.
  • Howson R, Huh WK, Ghaemmaghami S, Falvo JV, Bower K, Belle A, Dephoure N, Wykoff DD, Weissman JS, O'Shea EK. Construction, verification and experimental use of two epitope-tagged collections of budding yeast strains. Comp Funct Genomics. 2005;6(1-2):2-16. PubMed PMID: 18629296; PubMed Central PMCID: PMC2448600.
  • Ghaemmaghami S, Huh WK, Bower K, Howson RW, Belle A, Dephoure N, O'Shea EK, Weissman JS. Global analysis of protein expression in yeast. Nature. 2003 Oct 16;425(6959):737-41. PubMed PMID: 14562106.
  • Powell KD, Ghaemmaghami S, Wang MZ, Ma L, Oas TG, Fitzgerald MC. A general mass spectrometry-based assay for the quantitation of protein-ligand binding interactions in solution. J Am Chem Soc. 2002 Sep 4;124(35):10256-7. PubMed PMID: 12197709.
  • Ghaemmaghami S, Oas TG. Quantitative protein stability measurement in vivo. Nat Struct Biol. 2001 Oct;8(10):879-82. PubMed PMID: 11573094.
  • Ghaemmaghami S, Fitzgerald MC, Oas TG. A quantitative, high-throughput screen for protein stability. Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8296-301. PubMed PMID: 10890887; PubMed Central PMCID: PMC26941.
  • Ghaemmaghami S, Word JM, Burton RE, Richardson JS, Oas TG. Folding kinetics of a fluorescent variant of monomeric lambda repressor. Biochemistry. 1998 Jun 23;37(25):9179-85. PubMed PMID: 9636065.