Epithelial tissues are specialized to perform any of several functions: They can be protective, like the skin, which shields the body from the environment. They can be absorptive, like the lining of the intestine, which takes up nutrients from the lumen. They can be secretory, like glands that discharge hormones into the bloodstream.
All these functions rely on tissue impermeability; a leaky tissue can't form a protective barrier or regulate the transport of factors like nutrients and hormones. The cells that make up epithelial tissues are therefore tightly packed together. This arrangement must somehow be maintained even though individual cell behaviours, in particular division, can be disruptive to the local environment. (One could imagine this as a brick wall not only having to make space for more bricks, but also managing to do it without letting any air pass through.)
A failure in epithelial tissue permeability might not only disrupt function. Disorganization is also suspected to facilitate cancer. The importance of this possibility is underscored by the finding that 80-90% of all human tumors derive from epithelial cells.
The Bergstralh lab is therefore interested in two big questions: Firstly, how is epithelial integrity established and maintained? Secondly, what happens when it isn't?
We are using multiple model systems, including Drosophila melanogaster and cultured mammalian intestinal organoids, to address these questions.
Click here for a full list of publications.
- Finegan TM and Bergstralh DT. 2019. Division orientation in tissues: Disentangling shape and mechanical forces. Cell Cycle 18(11):1187-1198.
- Finegan TM, Na D, Cammarota C, Skeeters AV, Dawney NS, Oakes PW, Fletcher AG, and Bergstralh DT. 2018/19. Cover Article - Tissue tension and not interphase cell shape determines cell division orientation in the Drosophila follicular epithelium. The EMBO Journal e100072.
- Preview articles: ‘Oriented Cell Division: The Pull of the Pole,’ by G. Scepanovic and R. Fernandez-Gonzalez in the December 2018 issue of Developmental Cell and ‘Getting into shape: tissue tension drives oriented cell divisions during organogenesis’ by L. Manning and M. Peifer in the February 2019 issue of The EMBO Journal.
- Bergstralh DT, Lovegrove HE, Kujawiak I, Dawney NS, Zhu J, Cooper S, Zhang R, and St Johnston D. 2016. Highlighted Article - Pins is not required for spindle orientation in the Drosophila imaginal wing. Development 143(14):2573-2581.
- Bergstralh DT*, Lovegrove HE*, and St Johnston D. 2015. A mechanism for reintegrating misplaced cells into epithelial monolayers (* equal contribution). Nature Cell Biology.17(11):1497-503.
- Bergstralh DT and St Johnston D. 2014. Spindle Orientation: What if it Goes Wrong? Seminars Cell Dev. Biol.34C:140-145.
- Haack T*, Bergstralh DT*, and St Johnston D. 2013. Damage to the Drosophila follicle cell epithelium produces “false clones” with apparent polarity phenotypes. (* equal contribution) Biology Open.2(12):1313-20.
- Bergstralh DT, Haack T, and St Johnston D. 2013. Orientation and Epithelial Polarity: Intersecting Pathways. Phil. Trans. of the Royal Soc. B.368(1629):20130291.
- Bergstralh DT, Lovegrove HE, and St Johnston D. 2013. Discs large links spindle orientation to apical-basal polarity in Drosophila epithelia. Current Biology.23(17):1707-12.
- Bergstralh DT and St Johnston D. 2012. Epithelial Cell Polarity: What Flies Can Teach Us About Cancer. Essays Biochem.53(1):129-40.