Papers
Klar Functions in Nuclear Migration and Droplet Transport
M. A. Welte, S. P. Gross, M. Postner, S. M. Block, E. F. Wieschaus
(1998). "Developmental
regulation of vesicle transport in Drosophila embryos: forces and kinetics" Cell
92:547-557. Abstract
Klar mutations disrupt clouding in the early embryo because lipid droplets
fail to move out to the periphery (top panels). Klar mutations also
interfere with nuclear migration during eye development (lower panels). In
the developing eye disk, the nuclei of the photoreceptor cells are normally
positioned apically. In klar mutant animals, these nuclei accumulate on the
other, basal side of the tissue.
The role of Klar in eye development appears analogous to its role in
droplet transport. Both transport processes reverse net transport with precise
timing, and Klar is necessary for the switch from net basal to net apical
transport. In both cases, apical transport requires the minus-end motor cytoplasmic
dynein. Thus, Klar directly or indirectly regulates this universal eukaryotic
motor.